Data analysis chromatography

Determination of content in a sample with standard solutions (external standards)

Steps to determine the content in the sample:

  1. Prepare dilutions of the analyte solution as standards
  2. Measure standards
  3. Create calibration curve
  4. Measure sample
  5. Calculate the content of the sample from the analysis function

Calibration of a substance (analyte)
Fig 2
Measurement in a sample (mixture with other substances)

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About the learning unit


  • Prof. Reiner Salzer
  • Dr. Steffen Thiele
  • Dr. Astrid Zuern
  • Cordelia Carpenter